DIPG-01. LIN28B EXPRESSION IN DIFFUSE MIDLINE GLIOMA

Abstract INTRODUCTION Diffuse midline glioma (DMG) is the most lethal of all childhood cancers. LIN28B an RNA binding protein expressed in a variety cancers, which suppresses let-7 family microRNAs, turn plethora oncogenes. However, role DMG has not yet been explored. Therefore, we sought to determine pattern expression and potential function using rare cell lines. METHODS All studies were performed on lines (n=6) controls (normal human astrocytes n=1, neural stem cells n=1). RNA-Seq was single-read sequencing at 50bp Illumina NextSeq 500 Sequencing System. Cells then treated with DMSO or inhibitor, LI71, submitted functional vitro studies, as well Western Blot analysis. Functional pathways analysis subsequently resulting gene values Ingenuity Pathways Analysis (Qiagen). RESULTS Differential demonstrated across lines, expressing greater levels compared controls. inhibition small molecule inhibitor LI71 resulted decreased proliferation migration, vitro. A total 946 differentially genes identified between treatment groups (FC>2 <-2, p<0.05). implicated Cancer Neurological Disease top disease processes, Cellular Movement molecular process LIN28B. Further, inactivation Myc signaling LI71. CONCLUSIONS Our data demonstrates increased controls, oncogenic effects migration. Further investigation LIN28B-let7 axis warranted order further explore novel therapeutic target DMG.